Part:BBa_K4790061:Design
ASR1(node 59)-W120R
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 799
Illegal PstI site found at 100
Illegal PstI site found at 232 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 799
Illegal NheI site found at 721
Illegal PstI site found at 100
Illegal PstI site found at 232 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 799
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 799
Illegal PstI site found at 100
Illegal PstI site found at 232 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 799
Illegal PstI site found at 100
Illegal PstI site found at 232 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We should select direction of mutation which is predicted the highest possibility. Mutation should refer to Codon preference in E.coli.
Source
It does not come from any genomic sequence. Node 59 comes from Ancestral Sequence Reconstruction (ASR). The mutation site is predicted by machine learning.
References
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